Optimized method for dna extraction and PCR amplification in aroeira tree

Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies endangered populations. The different molecular techniques involve evaluation many individuals; therefore, it is crucial to have fast, efficient, inexpensive methods DNA extraction. Given importance Aroeira (Myracrodruon urundeuva Fr. All.) pertinent optimize a protocol that allows obtainment intact pure DNA, aiming assist this species threatened with extinction. Thus, study aimed compare five extraction methods: Dellaporta et al. (1983), Doyle (1987) modified, Ferreira Grattapaglia (1995), Romano Brasileiro (2015), Khanuja (1999) most efficient M. urundeuva. modified proposed by (1987), using 100 mg leaf tissue 6 µl β-mercaptoethanol was presented sharpest bands after electrophoresis reactions amplification employing Polymerase Chain Reaction (PCR). Therefore, suggested use described from young leaves carry out involving markers.